During your experiment, if you have any problem, you can call the local contact (dedicated business phone, or personal phone number if available) from 7am to 10 pm. If technical problems occur (vacuum, mechanical or electrical failure), do not try to fix them by yourself without agreement from the local contact. In case of emergency, call the hall operator (EHO) directly (phone: 25-25) or the ESRF control room (phone: 24-90).

In any case, you are not allowed to modify electric settings or try to fix electrical devices: if any action is necessary, it will be undertaken by the local contact himself or the technician on duty during the week-end. However, for a general electric power problem, you can contact the hall operator (phone: 25-25).

 

Sample preparation

 A preparation room at 20°C is available for the sample preparation. It includes

  • Leica microscopes with cold light sources, and a Visualization Bench for automated imaging of 96-well plates.

  • cryo-tools (crystalcap HT, crystalwand magnetic, vial clamp), foam bowls, gloves and other accessories for samples flash freezing and handling.

  • ESRF-EMBL standard baskets for the FIP sample changer robot.

  • A high pressure cell (up to 15 bars). It can be used to introduce in your crystal Krypton gas (the only one available on the beamline) or any non hazardous gaz (bring a bottle with you) prior to freezing. It requires that crystals are mounted on Hampton CrystalCap Copper (bottom one in this picture).

  • A 4°C fridge and a -20°C freezer.

  • A 20°C vibration-free incubator for crystallization trays storage.

 

No chemical solvent, heavy atom salt, gas (except Krypton) or acid are available. So bring any solution you may need with you, and do not forget to get it back at the end of the experiment as well as crystals. Special yellow bins are provided for specific waste (biological matters, sharp and pointed tools, needles, .......).

 

Sample mounting

Frozen sample holders (caps) can be mounted manually on the goniometer head. Alternatively, samples holders on their cryo-vial, stored in ESRF-EMBL baskets, can be placed in the sample changer Dewar (3 baskets at a time) for automated mounting with the FIP sample changer.

In case of crystals frozen in propane, mounting the crystal on the goniometer is simple: put the tube full of solid propane on the goniometer head and wait for the phase change (solid propane-liquid propane) and then remove the tube. The same operation is possible with the sample changer (select the “propane” mode in the graphical interface).

 

Data collection and data storage

Data are stored on NICE as well as locally. 

Data collection is controlled by an UNIX station with large disk space. As a consequence, at least one person per experiment must have a good knowledge of UNIX system.

 

Data reduction

 Data reduction can be performed on site. Available software are XDS, xdsme and MOSFLM. Usual data processing suites (CCP4, Phenix) are also available.